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KMID : 0381919970270020189
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Korean Journal of Microscopy
1997 Volume.27 No. 2 p.189 ~ p.196
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Ultrastructural Study of Induced Otic Vesicle from Isolated Xenopus Presumptive Ectoderm
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Yoon Chun-Sik
Kim Hong-Duck
Cheong Seon-Woo
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Abstract
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The ultrastrucures of Xenopus otic vesicle from normal embryo (st. 43) and induced otic vesicle from animal cap assay with Activin A were compared. Activin A was applied to the presumptive ectoderm at various concentrations for three days at $20^{\circ}C$. The results were almost identical to the reference studies, but it was found that the otic vesicle was induced at the concentration of 10 ng/ml in to)v rate. This otic vesicle may be secondarily induced by the neural tissue showed commonly at the concentration of 10 ng/ml. Otoliths were observed as three or four-axis crystaline bodies in the lumen of otic vesicle. In electron micrograph of the normal embryo, two kinds of microvilli were observed on the apical position of hair cells. One was small and common, the other was large-sized and sparsely distributed. In both cell of otic vesicle, mitochondria, golgi apparatus and multivesicular body were rich, so, they showed a lot of similarities in ultrastructure. However, the otolith was not found and microvilli were overexpressed in the otic vesicle induced by Activin A.
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KEYWORD
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Activin A, Otic Vesicle, Xenopus laevis, Animal Cap Assay, Electron Microscopy
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